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Sampling Techniques

Tim Hollibaugh shows the difference in a sample taken in surface water compared to one taken at the fluorescence maximum at 17 meters (see figure). Look carefully – the bottle on the left (surface) is clear while the bottle on the right has a greenish tinge from millions of tiny plants (the "Mickey Mouse" organism, Picocystis).
Erin Biers prepares to collect water samples from a Niskin Bottle. This device uses a spring-loaded trigger to close the end caps and collect water from a specific depth in the lake. A weighted "messenger" is sent down the hydrographic wire to "fire" the bottle after a short rinsing time. Niskin sampler
Sampling bottle
Gary Lecleir demonstrates how a remotely triggered sampling bottle works. This model, called a Van Dorn bottle, is triggered by a weight that slides down the rope used to lower the bottle. When triggered, the plugs at the ends are pulled together by a spring, closing the bottle and capturing the enclosed volume (2 liters).
Tim Hollibaugh demonstrates the profiling instrument used to collect the data shown in the figure. For you technophiles, the basic unit is a standard shallow water SeaBird SBE 19 CTD with a SeaTech fluorometer and a LiCor 2p PAR sensor. A special conductivity sensor has to be used in Mono Lake’s high salinity environment. CTD profiler
Plankton sampler
Tim Hollibaugh demonstrates a special device made for sampling thin layers of plankton ("plates") in Mono Lake that form at the interfaces between water layers. The hose attached to the top is connected to a peristaltic pump. Hollibaugh is pointing to a slot that water is drawn through the when the pump is running.
Dr. Sally MacIntyre demonstrates the operation of an "acoustic Doppler current profiler" (ADCP), used to map currents in Mono Lake. This device sends out sonar beams in 3 directions and uses the differences in the signals to map the vertical distribution of currents in the lake. ADCP
Whaler
Boston whaler, crew, samples, and gear returning from a successful sampling mission.
     
 
photos courtesy of Arya Degenhardt, Mono Lake Committee

07-Apr-2005
Web design by Wade Sheldon, Univ. of Georgia
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This material is based upon work supported by the National Science Foundation under grants MCB 99-77886, MCB 99-77892, and MCB 99-77901. Any opinions, findings, conclusions, or recommendations expressed in the material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

NSF